Supplementary data for "The subgenual organ complex in stick insects: Functional morphology and mechanical coupling of a complex mechanosensory organ"

µCT-scans of the upper tibial regions of the foreleg (T1) and the midleg (T2) of Ramulus artemis (Westwood, 1859), Carausius morosus (Sinéty, 1901), and Sipyloidea sipylus (Westwood, 1859). For use of scans, please cite the following publication:

Strauß, J., Moritz, L. & Rühr, P.T. (2021): The subgenual organ complex in stick insects: Functional morphology and mechanical coupling of a complex mechanosensory organ. Frontiers in Ecology and Evolution (Research Topic “Evolutionary Biomechanics of Sound Production and Reception”). doi: 10.3389/fevo.2021.632493.

All scans were performed with a commercial μCT desktop system (Skyscan 1272, Bruker microCT, Kontich, Belgium) at the Zoological Research Museum Alexander Koenig, Leibniz Institute for Animal Biodiversity, Bonn, Germany.

µCT scan settings of all samples:

Ramulus artemis:

tube voltage = 30 kV

ube current = 200 μA

target = tungsten

no filter

total sample rotation = 360°

angular step size = 0.2°

exposure time = 1980 ms

binning = 1x1

averaging = 8

random movement = 15 px

voxel size = 1.8 μm

fixation: Bouin's solution (24 hours)

staining: 0.3% PTA (21 days)

storage: 70% EtOH

surrounding medium in scan: 70% EtOH

filenames: Ramulus_artemis_T1.tif; Ramulus_artemis_T2.tif

Carausius morosus:

tube voltage = 29 kV

ube current = 200 μA

target = tungsten

no filter

total sample rotation = 360°

angular step size = 0.2°

exposure time = 1900 ms

binning = 1x1

averaging = 5

random movement = 15 px

voxel size = 1.0 μm

fixation: Bouin's solution (24 hours)

staining: 0.3% PTA (21 days)

storage: 70% EtOH

surrounding medium in scan: 70% EtOH

filenames: Carausius_morosus_T1.tif; Carausius_morosus_T2.tif

Sipyloidea sipylus:

tube voltage = 29 kV

ube current = 200 μA

target = tungsten

no filter

total sample rotation = 360°

angular step size = 0.2°

exposure time = 1900 ms

binning = 1x1

averaging = 7

random movement = 15 px

voxel size = 1.8 μm

fixation: Bouin's solution (24 hours)

staining: 0.3% PTA (21 days)

storage: 70% EtOH

surrounding medium in scan: 70% EtOH

filenames: Sipyloidea_sipylus_T1.tif; Sipyloidea_sipylus_T2.tif