File S1 - <i>Mycobacterium bovis</i> BCG Triggered MyD88 Induces miR-124 Feedback Negatively Regulates Immune Response in Alveolar Epithelial Cells

Supporting table and figures. Table S1. Potential targets of miR-124 in TLR signaling pathway predicted by bioinformatics tools. Figure S1. miR-124 regulates IFN-γ expression. The expression of IFN-γ was detected by qRT-PCR in A549 cells transfected with pcDNA3.1, miR-124 nc, miR-124 mimic or miR-124 inhibitor followed by infected without (A) or with BCG (B). Compared with pcDNA3.1 group *: p<0.05. Data represented the mean ± SD from three independent triplicated experiments (N = 6). Figure S2. miR-124 regulates IL-1α expression. The expression of IL-1α was detected by qRT-PCR in A549 cells transfected with pcDNA3.1, miR-124 nc, miR-124 mimic or miR-124 inhibitor followed by infected without (A) or with BCG (B). Compared with pcDNA3.1 group *: p<0.05. Data represented the mean ± SD from three independent triplicated experiments (N = 6). Figure S3. miR-124 regulates IL-8 expression. The expression of IL-8 was detected by qRT-PCR in A549 cells transfected with pcDNA3.1, miR-124 nc, miR-124 mimic or miR-124 inhibitor followed by infected without (A) or with BCG (B). Compared with pcDNA3.1 group *: p<0.05. Data represented the mean ± SD from three independent triplicated experiments (N = 6). Figure S4. miR-124 regulates IL-12α expression. The expression of IL-12α was detected by qRT-PCR in A549 cells transfected with pcDNA3.1, miR-124 nc, miR-124 mimic or miR-124 inhibitor followed by infected without (A) or with BCG (B). Compared with pcDNA3.1 group *: p<0.05. Data represented the mean ± SD from three independent triplicated experiments (N = 6). (PDF)