Bulk RNA sequencing on CD24+CD29+SSCs isolated from femurs of mice exposed to Low fat diet and High fat diet for 4 weeks. Bulk RNA sequencing on CD24+CD29+SSCs isolated from femurs of mice exposed to Low fat diet and High fat diet for 4 weeks

To understand how High fat diet and Low fat diet exposure alters gene expression profile in CD24+CD29+SSC present in the bone. Overall design: Perform FACS isolation of CD24+CD29+SSCs from mouse femurs after 4 weeks of diet exposure and subjecting them to RNA sequencing