Next Generation Sequencing facilitates identification of differentially expressed genes in circulating and hepatic CD161+CD8+T cells (CD161P), CD161-CD8+T cells (CD161N) and MAIT cells from healthy donors(HD) and HBV-infected patients

We enriched circulating and hepatic CD161N, CD161P and MAIT cells through flow sorting from heathy donors and HBV-infected patients to perform next-generation sequencing (NGS). Illumina mRNA-seq sample preparation kit was used to perform paired-end library sequencing with Illumina novaseq 6000 and sequence analysis was determined using the Illumina data analysis pipeline. Based on the comparison results, we performed the GO and KEGG pathway enrichment analysis. That might suggest the different status of CD161N,CD161P and MAITcells from healthy donors and HBV-infected patients. Differential gene expression between circulating and hepatic CD161N, CD161P and MAIT cells from healthy donors and HBV-infected patients