Vessel Forming Mesenchymal Stromal Cell Populations

We have identified two vessel forming mesenchymal stromal cell populations. Population 1 (P1) is CD45-Ter119-Tie2+PDGFRa-CD31+CD105highSca1low and gives rise to stunted vessels (incomplete tubular structures) in a transplant setting, and Population 2 (P2) which is CD45-Ter119-Tie2+PDGFRa+CD31-CD105lowSca1high and forms stunted vessels and fat. To test the homogeneity and signal interactions of these populations we performed 10X single cell RNA-sequencing on them. Overall design: Two vessel forming mesenchymal stromal cell populations were FACS sorted according to their surface markers, P1 is CD45-Ter119-Tie2+PDGFRa-CD31+CD105highSca1low and P2 is CD45-Ter119-Tie2+PDGFRa+CD31-CD105lowSca1high,10x single cell RNA-sequencing were performed after sorting.