Transcriptomics of FGF6-stimulated mouse brown preadipocytes

Uncoupling protein-1 (UCP1) plays a central role in energy dissipation in brown adipose tissue (BAT). Using high-throughput library screening of secreted peptides, we identified two fibroblast growth factors (FGF), FGF6 and FGF9, as novel and potent inducers of UCP1 expression in adipocytes and preadipocytes. Here we show the transcriptome of FGF6-stimulated mouse brown preadipocytes. Immortalized murine brown preadipocytes were treated with vehicle or FGF6 for 4, 8, and 24 hours. Total RNA was isolated using Direct-zol™ RNA miniprep Kits (Zymo Research, Irvine, CA). High-throughput sequencing was performed using a HiSeq 4000 instrument (Illumina) at BGI Americas.