Size Fractionated Chlorophyll Measurements from R/V Tangaroa TAN1810 in the Chatham Rise (Subtropical and Sub-Antarctic waters off of New Zealand) from October to November 2018 (Salp Food Web Ecology project)

This dataset provides size-fractionated (SF) chlorophyll measurements taken across the subtropical front east of New Zealand in October and November 2018. The dataset provides depth-resolved measurements of SF chlorophyll for 0.2 µm, 2 µm and 20 µm sized cells for locations in the subtropical and subantarctic-influenced locations of the subtropical front. Samples for size fractionated chlorophyll a (SF Chla) were analyzed by first gravity filtering a sample (250 mL) through a 20 µm 47-mm polycarbonate filter, then with low vacuum pressure, filtering sequentially through a 2 µm and a 0.2 µm filter. Filters were then folded, placed in 1.5 mL cryovials and frozen (-80 °C) until analysis (Gutiérrez‐Rodríguez et al., 2020). Chla and acidified phaeopigment-a concentrations were measured (within 3 months) using ice-cold 90% acetone extraction by spectrofluorometric methods (APHA 10200 H) on a Varian Cary Eclipse fluorescence spectrophotometer (Rice et al., 2012). In addition to serving as the chlorophyll measurements for size specific C:Chl ratios, size fractionated chlorophyll measurements are crucial in estimating chlorophyll concentrations for various sized autotroph cells. The summed SF chlorophyll is also provided in this dataset as comparison to total chlorophyll sampled in this region. Andrés Gutierrez Rodriguez and his lab were responsible for collection and analysis of this dataset.

本数据集提供了2018年10月和11月在新西兰东部的亚热带锋面区域进行的叶绿素大小分数（SF）测量。数据集提供了亚热带锋面及受亚南极地区影响区域的0.2微米、2微米和20微米尺寸细胞的SF叶绿素深度解析测量。大小分数化叶绿素a（SF Chla）样品经首先通过20微米47毫米聚碳酸酯滤膜进行重力过滤（250毫升样品），随后在低压下依次通过2微米和0.2微米滤膜进行过滤。随后，滤膜被折叠，放入1.5毫升冷冻管中并冷冻至-80°C，直至分析（Gutiérrez-Rodríguez等，2020年）。叶绿素a和酸化叶绿素a浓度（APHA 10200 H）在Varian Cary Eclipse荧光分光光度计上使用冰冷的90%乙腈提取（Rice等，2012年）进行测量。除了作为特定大小C:Chl比率的叶绿素测量外，大小分数化叶绿素测量对于估算不同尺寸的自养细胞叶绿素浓度至关重要。此外，数据集中还提供了总和的SF叶绿素作为该区域采样总叶绿素的比较。Andrés Gutierrez Rodriguez及其实验室负责了该数据集的收集和分析。