The efficiency of reprogramming CD34+ cells beginning from 4 ml of blood or less.

Single, 8 ml vials of blood were fractionated from a subset of the donors from Table 3 to represent half or one-quarter of a whole vial. PBMCs were isolated and CD34+ cells purified from them for use in the reprogramming method described here. Asterisks indicate the generation of iPSCs from CD34+ cells isolated fresh from blood while all other trials were performed from CD34+ cells isolated from PBMCs that were frozen down immediately after isolation. Plasmid combination Set 2 expressing L-myc was used for transfection on cells from each of these donors. N indicates the number of independent reprogramming trials performed. Reprogramming efficiencies were averaged where N>1.