Comparison of avibactam inhibition kinetics and avibactam active site interaction.

athe chirality of avibactam tertiary N1 atombthe direction of N6 inwards towards the carbonyl carbon or away towards the solventcthe number of hydrogen bonds made with the oxygen atoms of the sulfatedhydrogen bonds formed with the nitrogen N6 of avibactamehydrogen bond formed with the sulfate oxygen adjacent the N6 atomfpresence of an active site water hydrogen bonding to an oxygen atom of the sulfate and positioned above the plane of the 3 oxygen atoms to potentially initiate a nucleophilic attack (as in Fig 1)gsulfate moiety is within hydrogen bonding distance of a positively charged side chain (Arg (R) or Lys (K)).Comparison of avibactam inhibition kinetics and avibactam active site interaction.