RNA Sequencing of Cardiac in STZ-induced Rat model Uncovers Potential Target of Diabetic cardiomyopathy

Diabetic cardiomyopathy (DCM) is one of the major causes of heart failure in diabetic patients, but its pathogenesis remains unclear. Long non-coding RNAs (lncRNAs) are involved in the development of various cardiovascular diseases, but is little known in DCM. We build diabetic cardiomyopathy (DCM) rat model and investigated the genome-wide expression profiling of cardiac lncRNAs and mRNAs in rat model with and without DCM by RNA sequencing, to uncovers potential path mechanisms target of DCM. Overall design: Sprague Dawley (SD) rats (8 weeks old, male) injection with a single dose of 50 mg/kg streptozocin (STZ) to build the DCM rat model. Total RNA was isolated from left ventricular tissue of SD rats at 96 days after STZ injection (Control sample, n=5 and Model sample, n=5). The RNA-sequencing were performed on the Illumina sequencing platform (HiSeqTM 2500). Further bioinformatics analysis included co-expression, ceRNA network and functional enrichment analysis of deregulated lncRNAs and mRNAs were performed.