Structural mechanism of H3K27 demethylation and crosstalk with heterochromatin markers - Lin et al

Data S1. Data files, related to Figure 2.
(A-F) Uncropped electrophoretic mobility shift assay (EMSA) gels showing nucleosome binding by KDM6B.
(G-H) Uncropped western blot images of nucleosome demethylation assays.
Data S2. Data files, related to Figures 3, 4 and 5.
(A-J) Uncropped EMSA gels showing nucleosome binding by KDM6B and its mutants.
(K-L) Uncropped western blot images of nucleosome demethylation assays performed with KDM6B and its mutants.
Data S3. Data files, related to Figure 6.
(A-D) Uncropped western blot images of nucleosome demethylation assays performed with KDM6B and gH1.5.
(E-F) Uncropped western blot images of nucleosome demethylation assays performed with KDM6B using 20N20 and 20N20-H2AK119C-DCA-ub1 nucleosomes as substrates.
(G-H) Uncropped western blot images of single-turnover nucleosome demethylation assays performed by KDM6B using 20N20 and 20N20-H2AK119C-DCA-ub1 nucleosomes as substrates.
Data S4. Data files, related to Figures S1, S2 and S9.
(A-D) Uncropped gels of purified (A) KDM6B, (B) KDM6B mutants, KDM6B-nucleosome complex, (C) gH1.5, and (D) nucleosome substrates.
(E) Uncropped EMSA gels showing KDM6B binding to 1N1 and 20N20 nucleosomes.
Data S5. Data files, related to Figure S9.
(A-B) Uncropped western blot images of time-course nucleosome demethylation assays performed by KDM6B using 20N20 and 20N20-H2AK119C-DCA-ub1 nucleosomes as substrates. (A) H3K27me3, (B) H3
(C-D) Uncropped western blot images of time-course nucleosome demethylation assays performed by KDM6B using 20N20 and 20N20-H2AK119ub1 nucleosomes as substrates. (C) H3K27me3, (D) H3
(E-F) Uncropped western blot images of single-turnover nucleosome demethylation assays performed by KDM6B using 20N20 and 20N20-H2AK119ub1 nucleosomes as substrates. (E) H3K27me3, (F) H3
Data S6. Excel file containing quantification data from EMSA experiments related to Figure 2B.
Data S7. Excel file containing raw data for FRET experiments, related to Figures 2D, 3D, 4D and 5D.
Data S8. Excel file containing ImageJ-based quantification data for nucleosome demethylation assays, related to Figure 2C.
Data S9. Excel file containing ImageJ-based quantification data for nucleosome demethylation assays, related to Figures 3C, 4C and 5C.
Data S10. Excel file containing ImageJ-based quantification data for nucleosome demethylation assays, related to Figure 6C.
Data S11. Excel file containing ImageJ-based quantification data for nucleosome demethylation assays, related to Figure 6F.
Data S12. Excel file containing ImageJ-based quantification data for nucleosome demethylation assays, related to Figure 6G.
Data S13. Excel file containing ImageJ-based quantification data for nucleosome demethylation assays, related to Figure S9C.
Data S14. Excel file containing ImageJ-based quantification data for nucleosome demethylation assays, related to Figure S9D.
Data S15. Excel file containing ImageJ-based quantification data for nucleosome demethylation assays, related to Figure S9E.