Marine microbial degradation of water-soluble crude oil components - nutrient and total organic carbon data

This dataset includes the auxiliary information for the marine microbial degradation of water-soluble crude oil components experiment, which includes nutrients and total organic carbon concentrations collected at all time points of the experiment. See below for experiment details. Crude oil-derived polar compounds are receiving increasing attention due to their higher proportion in weathered oil, higher water solubility and potential harmful environmental impacts. However, these compounds are not amenable to GC-based analytical methods. Current knowledge on the characteristics and weathering patterns of oil-derived polar compounds relies on Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). This study aimed to couple, FT-ICR-MS analysis with emerging metabolomics techniques to characterize the metabolic products of petroleum weathering. Natural marine bacteria consortia were exposed to crude oil-derived compounds dissolved in seawater in a lab incubation study. Three sets of controls were used: (1) sterile seawater with oil-derived water-soluble compounds; (2) sterile seawater with natural marine bacteria and succinic acid, which was used as a simple carbon source instead of crude oil; and (3) seawater alone with natural marine bacteria. All incubation sets were supplemented with nutrients and kept in dark at room temperature for 14 days.