Figure S1 - Recycling of the High Valence States of Heme Proteins by Cysteine Residues of Thimet-Oligopeptidase

Effect of different concentrations of TCEP on the percentage of thiol content of TOP and protein structure. A – Percentage of reduced cysteine residues of 10 μM TOP treated with different concentrations of TCEP at two pH values. B – Circular dichroism (CD) spectra of TOP reduced by TCEP. The gray line represents the CD spectrum of catalytically active TOP reduced by 1 mM TCEP and the black line represents the CD spectrum inactive TOP treated with 10 mM TCEP. (DOCX)