Molecular diversity and developmental mechanisms of ventral midbrain GABAergic neurons

single-cell RNA sequencing data (plate-based CelSeq2) of developing mouse ventral midbrain GABAergic neurons. vmbGABA series contains E16 and P0 VGAT mouse ventral midbrain and E16 and P0 VGAT/PITX3 co-expressing mouse ventral midbrain. These cells were isolated from VGAT-Cre:Ai14:Pitx3-GFP mice using FACS sorting for td-tomato signal to isolate GABAergic neurons and td-tomato + GFP signal to isolate the co-expressing population. single-cell RNA sequencing data (plate-based CelSeq2) of isolated GABAergic neurons in developing mouse ventral midbrain.发育中小鼠腹侧中脑γ-氨基丁酸能神经元（GABAergic neurons）的单细胞RNA测序（single-cell RNA sequencing）数据，测序采用基于微孔板的CelSeq2平台。vmbGABA数据集包含胚胎第16天（E16）及出生后第0天（P0）的VGAT阳性小鼠腹侧中脑样本，以及胚胎第16天、出生后第0天同时表达VGAT与PITX3的小鼠腹侧中脑样本。所有细胞均取材于VGAT-Cre:Ai14:Pitx3-GFP转基因小鼠，通过荧光激活细胞分选（fluorescence-activated cell sorting, FACS）完成分离：以td-tomato信号为标记分选γ-氨基丁酸能神经元，以td-tomato与GFP联合信号为标记分选共表达双基因的细胞群。本数据集涵盖经分离纯化的发育中小鼠腹侧中脑γ-氨基丁酸能神经元的单细胞RNA测序数据，测序采用基于微孔板的CelSeq2平台。