Flow Cytometry data

Raw flow cytometry data of microbial community experiment focused on switching between r- and K- selection. The files are in the Flow Cytometry Standard (FCS) format. They are compressed and packed into a tar-archive using the tar -a option.<br>For each sample, the bacterial density was quantified using flow cytometry (BC Accuri C6). In brief, the bacterial communities were diluted in 0.1x TE buffer, mixed with 2x SYBR Green II RNA gel stain (ThermoFisher Scientific) and incubated in the dark at room temperature for 15 minutes. Then, each sample was measured for 2.5 minutes at 35 uL/min with an FL1-H (533/30 nm) threshold of 3000. We gated the bacterial population as those events with an FL1-A &gt; 10⁴ and FSC-A &lt; 10⁵.<br>