Properties of V2 region monoclonal antibodies.

RCM-rgp120, indicates the ability of MAbs to bind to denatured (reduced and carboxymethylated) gp120. HIV rgp120 binding, indicates the ability of MAbs to bind to recombinant gp120 from the MN, IIIB and JRCSF strains of HIV in an ELISA format. CD4 blocking represents a summary of the data provided in Supplemental Figure S1. Neutralization data represents the MAb concentration (µg/mL) required for 50% inhibition (IC50) of infectivity by the MN-3 strain of HIV-1 in the TZM-bl neutralization assay (Materials and Methods) with the exception of the 6E10 MAb (*) where neutralization titers were measured using the IIIB strain of gp120 as described previously [27]. Controls for the assays (italic) included MAbs 1026, b12 and PG9. ND, indicates not done. Epitope localization, summarizes the minimal gp120 structure required for antibody binding based on MAb reactivity with the gp120 fragments shown in Figure 1.