Uncoupling of transcription and cytodifferentiation in mouse spermatocytes with impaired meiosis

We use the cytologically well-defined Prdm9 mutant mouse as a model of developmental arrest to demonstrate that parallel programs of cellular differentiation and transcription can become dis-associated. By comparing cytological phenotype markers and transcriptomes in wild-type and mutant spermatocytes, we identified multiple instances of cellular and transcriptional uncoupling in Prdm9-/- mutants. Transcript abundance from mouse spermatocytes with wild-type, heterozygous, and homozygous mutant alleles of Prdm9, collected at three time points with replicates.我们以细胞学特征明确的Prdm9突变小鼠作为发育停滞模型，证实细胞分化与转录的平行调控程序可发生解偶联。通过对比野生型与突变型精母细胞的细胞学表型标记物及转录组，我们在Prdm9-/-突变体中鉴定出多例细胞与转录解偶联现象。本数据集包含携带Prdm9野生型、杂合突变及纯合突变等位基因的小鼠精母细胞的转录本丰度信息，样本于三个时间点采集并设置生物学重复。