Translation of Circular RNAs in Neural Retina

We performed Ribosome-protected mRNA fragment sequencing (Ribo-Seq) to determine if retinal circular RNAs are undergoing translation Lysates from rat retinal tissues were prepared using lysis buffer with or without detergent. Lysates were digested with RNase I, purified and further processed for isolation of ribosome-protected RNA fragments. The recovered RNA fragments were subjected to ribosomal RNA removal using Illumina Ribo-Zero Gold kit and the samples were sequenced using Illumina NextSeq 500.