Supplementary_data_8.csv

Overrepresentation of nORFs regulated by either Hsf1 or Sfp1 in GO BP terms. <br> For Hsf1 analysis, RNA-seq samples were from Ciccarelli et al. (SRA accession SRP437124). Hsf1 deletion strains were compared to wild type (WT) strains when exposed to heat shock conditions. For Sfp1 analysis, RNA-seq samples were from SRA accession SRP159150. In both cases, deletion strains were compared to WT strains. Differential expression was calculated using R package <em>DESeq2</em>, and ORFs were defined as differentially expressed if log fold change log(FC) &gt; 0.5 or log(FC) &lt; -0.5 and BH adjusted p-value &lt; 0.05. ChIP-exo data for Hsf1 and Sfp1 binding was taken from Rossi et al. and an ORF was labeled has having Hsf1 or Sfp1 binding if the TF was found within 200 bp upstream of the ORF’s transcription start site (TSS). Fisher’s exact test was performed to see if there is an association between an nORF in a GO biological process and being regulated by the TF. We define an nORF to be "in" a GO term if it has a GSEA enrichment for that GO term at FDR &lt;0.01. We defined an nORF as regulated by the TF if the nORF had evidence of the TF binding within 200 bp of the nORF’s promoter in ChIP-exo and has significantly downregulated expression in the TF deletion RNA-seq samples compared to the WT samples. BH p-value correction was performed for all GO terms tested. <br>