ATAC-seq on DHT treated/untreated prostate cell line RWPE1

Precise gene regulation is needed for the correct differentiation and function of cells. This is especially important when putting cells through stimuli that would change their characteristics. To understand the changes in chromatin accessibility of prostate cells when stimulating them with testosterone, we performed the ATAC-seq (Assay for Transposase Accessible Chromatin using sequencing). We have worked with the normal epithelial prostate cell line RWPE1, an heterogenous cell line composed of two subpopulations: WPE-stem (stem cell phenotype) and WPE-int (intermediate cellular subtype) and treated it with DHT. Our aim was to study haemoglobin gene regulation in prostate cells, and check whether changes in cell stimulus would lead to changes in chromatin accessibility in both haemoglobin loci. Nevertheless, the results obtained could be used to compare chromatin accessibility between DHT treated and untreated cells on any loci. ATAC-seq of the normal epithelial prostate cell line RWPE1 untreated (2 replicates) or treated with DHT (2 replicates)