Differential SP4 expression and HSP60 abundance in buccal swabs from patients with schizophrenia

Schizophrenia (SCZ) is heterogenous and polygenic, making it difficult to diagnose and treat. This is unsurprising as diagnostic criteria are solely based on behavioral markers. There is a critical need for easy-to-collect biomarkers that aid in treatment. To identify potential biomarkers, we recruited SCZ patients and controls. Using buccal cell lysates, we performed real-time quantitative PCR and identified significant differences in Sp4 mRNA between patients and controls. Targeted mass spectrometry identified increased heat shock protein 60 (HSP60) in SCZ samples. To determine the utility of Sp4 mRNA and HSP60 protein as biomarkers, we evaluated their relationship with symptom severity and aberrant cognitive processes. Correlational analyses revealed that elevated Sp4 mRNA and HSP60 protein define a subgroup of SCZ patients who demonstrate symptomology and poor memory. These data support buccal cell Sp4 mRNA and HSP60 protein as easy-to-collect, candidate SCZ biomarkers for a subset ..., We received explicit consent from our participants to publish the de-identified data in the public domain, and explain all data have codes with no link to names or identifying information., , # Differential SP4 expression and HSP60 abundance in buccal swabs from patients with schizophrenia

**Short title:** SP4 and HSP60 biomarkers in schizophrenia patients
**Journal:** *Science Advances* (submitted / revision package)
**Dataset DOI**:10.5061/dryad.6djh9w1fj.zip

## Overview

This README describes all data files associated with the manuscript, including raw experimental outputs, processed datasets, and a consolidated master summary dataset used for all statistical analyses. The dataset includes molecular, cognitive, and clinical measures derived from buccal swabs and behavioral assessments in patients with schizophrenia and control subjects.

All statistical analyses, normalization procedures, and model specifications are described in detail in the main text and the Materials and Methods section of the manuscript. No analysis scripts or proprietary analysis workspaces are provided. All datasets are supplied in non-proprietary CSV format with NA used to denote missing or non-...