Fine scale population divergence driven by adaptation to industrial pollutants

The federal Superfund site at New Bedford Harbor (Massachusetts, USA) is an example of an environment where pollution levels rose quickly and dramatically. Industrial waste containing polychlorinated biphenyls, heavy metals, and other organic pollutants was dumped into the harbor in the mid-20th century. The mummichog (Fundulus heteroclitus) is a widely distributed fish typically susceptible to polychlorinated biphenyl toxicity. However, the population in New Bedford Harbor is one of several that have evolved the ability to tolerate this category of toxicants. Constituents of the aryl hydrocarbon receptor system are linked to this adaptive pollution tolerance. Our population genetic analysis of 444 mummichogs from Massachusetts and Rhode Island estuaries using 55 SNP loci suggests that F. heteroclitus near New Bedford Harbor has large populations and restricted but meaningful levels of gene exchange among adjacent habitats. When comparing polluted to cleaner sites, we find strong eviden..., This dataset contains data from 55 SNPs from Fundulus heteroclitus collected from 11 habitats along the shores of Massachusetts and Rhode Island (USA). SNPs were assayed using a Fluidigm EP-1 system. , , # Fine-scale population divergence driven by adaptation to industrial pollutants

[https://doi.org/10.5061/dryad.n8pk0p327](https://doi.org/10.5061/dryad.n8pk0p327)

We analyzed 55 SNPs in 444 *Fundulus heteroclitus* collected from 11 habitat patches along the southern shores of Massachusetts and Rhode Island. These include habitats within the highly polluted Superfund site in and around New Bedford Harbor. Fish in these habitats have been shown to have evolved to tolerate high levels of polychlorinated biphenyl (PCB) pollution (Nacci et al, 1999). Several of the SNPs surveyed are within loci hypothesized to be important in PCB tolerance.

Nacci, D., et al. \"Adaptations of wild populations of the estuarine fish *Fundulus heteroclitus* to persistent environmental contaminants.\" *Marine Biology* 134 (1999): 9-17.

## Description of the data and file structure

Data are shared as a tabbed Microsoft Excel file.

#### **Tab 1**

contains raw data in GenAlEx format (Peakall and Smouse, 2006)....