Sodium Ion Changes in the Skin of Rats Under High-Salt Diet Conditions and the Potential Mechanism of the Skin Acting as a Sodium Ion Buffer

This dataset investigates the impact of a high-salt diet on sodium ion changes in the skin of rats and explores the role of the skin as a sodium ion buffer. The study includes detailed measurements of sodium and potassium ion concentrations in blood, urine, and skin, as well as analyses of glycosaminoglycans (GAGs), macrophage activity, lymphangiogenesis, and protein expressions related to the TonEBP/VEGF-C/VEGFR-3 pathway. Male SD rats were divided into five groups with varying dietary salt levels, including a transitional group shifting from high- to low-salt intake. Key findings include increased skin sodium levels and GAG concentrations under high-salt conditions, along with upregulated macrophage and lymphatic vessel markers. The dataset supports the hypothesis that the skin acts as a dynamic sodium reservoir, buffering blood pressure and sodium load, with lymphatic remodeling as a crucial mechanism. Data includes raw measurements, statistical analyses, and experimental protocols for sodium/potassium quantification, ELISA, immunofluorescence, and Western blotting, offering comprehensive insights into sodium homeostasis and related physiological pathways.