mRNA-seq of Rai1 ex 4 KO and wild-type control lines in mESC-differentiated NPCs and neurons. mRNA-seq of Rai1 ex 4 KO and wild-type control lines in mESC-differentiated NPCs and neurons

"Mouse 129-B13 ESCs were genetically modified using CRISPR-Cas9 with two guides to remove exon 4 of Rai1, single-cell sorted and expanded to establish modified cell lines. Additionally, cell lines derived from unsuccessfully modified lines were used as ""wild-type"" controls. 3 lines of each group were used as biological replicates. Samples were differentiated from mouse ESCs (Day 0) to neural progenitor cells (Day 8) and glutamatergic neurons (Day 12). NEBNext Poly(A) mRNA Magnetic Isolation Module was used to enrich mRNA. Nonsense-mediated decay was not observed in Rai1 ex 4 KOs."