Replication Data for: Tetrameric, active PKM2 inhibits IP3 receptors, potentially requiring GRP75 as an additional interaction partner

This dataset includes all the data integrated in the publication in Lemos et al., 2024, BBA-MCR and contains all data necessary to replicate the published figures. The project investigates the nature and the properties of the metabolic enzyme PKM2 in the regulation of IP3R-mediated Ca2+ signaling. Using both chemical and genetic methods, we provide evidence that the catalytical active, tetrameric form of PKM2 preferentially suppresses IP3R-mediated Ca2+ release. Moreover, our data strongly suggests that this inhibitory effect necessitates an additional cellular factor, which is most likely GRP75.
All info concerning the integrated data can be found in the attached "README-BBA final" file.