Genome-wide maps of chromatin state of H3K27ac in embryonic and neonatal cardiomyocytes of XMlc2-Cre;Dot1L mutants and controls.

Epigenetic enzymes play critical roles in embryogenesis by defining higher-order chromatin structures required for the establishment of organ-specific transcriptional networks. In this study we investigated how the landscape of the histone 3 lysine 27 acetylation in absence of the histone methyltransferase Dot1L in cardiomyocytes during development. We conducted chromatin immunoprecipitation experiments for H3K27ac in embryonic (E16.5) and neonatal (P1) cardiomyocytes from Dot1L cardiomyocyte-specific conditional knock out and control mice. Overall, we identified alteration in the enrichment of acetylation of H3K27 in regulatory regions associated wih genes essential for cardiac patterning and cardiomyocyte maturation. Overall design: H3K27ac ChIP-seq profiling were performed on FACS purified cardiomyocytes from XMlc2-Cre;Dot1L mutant and controls at E16.5 and P1.