Single-nucleus RNA-seq characterizes the cell types along the neuronal lineage in the adult human subependymal zone

The subependymal zone (SEZ), also known as the subventricular zone (SVZ), constitutes a neurogenic niche that persists during post-natal life. To investigate the cellular diversity of this brain region during human adulthood, we characterized the complete cellular niche of the adult human SEZ by single-nucleus RNA sequencing (snRNAseq), in youth and middle-aged adults. Single nuclei were isolated from SEZ frozen post-mortem tissue from 4 young (16-22 years) and 4 middle-aged (44-53 years) adults. DAPI positive events were collected by FACS and subjected to snRNA.