Identification and Quantitative Analysis of Cellular Proteins Affected by Treatment with Withaferin A using a SILAC-based Proteomics Approach

Withaferin A (WA) is a major bioactive compound isolated from the medicinal plant Withania somnifera Dunal, also known as ‘‘Ashwagandha’’. A number of published reports suggest various uses for WA including its function as an anti-inflammatory and anti-angiogenic drug molecule. The effects of WA at the molecular level in a cellular environment are not well understood. Knowledge of the molecular mechanism of action of WA could enhance its therapeutic value and may reveal novel pathways it may modulate.