Third instar larvae were dissected in PBS and fixed in 4% paraformaldehyde for 25 min at room temperature, then washed in PBST three times (each 10 min), blocked in blocking solution (PBST with 5% nor
Figs A-E. (A) Experimental design for gestational exposure to the prototypical AHR ligand (TCDD). (B) Body weight (grams) from naïve Ahr+/+, low- and high-dose ligand-exposed Ahr+/+, and Ahr-/- males
