Primers and PCR conditions used in this study.
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*Restriction sites (underlined) introduced in primers: GGTACC (KpnI), GGATCC (BamHI), ACTAGT (SpeI), and TCTAGA (XbaI). Default PCR conditions were: initial denaturation, 95°C/5 min; denaturation, 94°C/30 s; number of cycles, 30; and final extension, 72°C/7 min.
创建时间：
2015-12-02
