Additional file 3: of Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

Table S2. NGS Read Accounting of Sequenced LASSO E. coli ORF-eome Captures. The table shows raw sequencing output (HiSeq 1 × 50 bp platform) of PCR amplified multiplexed ORF-eome capture. Reads were first aligned to both E. coli genome and LASSO probe backbone sequence, then read mapped exclusively to genomic DNA was cleaned, filtered, and separated according to targets or non-targets (untargeted ORFs and intergenic regions). Also included are the median read depths of gDNA sequencing enrichment. (XLSX 9 kb)