Supplimentary file.pdf DNA ragmentation

Title:Raw data and uncropped gel images supporting “Novel Pyrimidine-Substituted Chalcones: In Vitro Antioxidant Properties and Cytotoxic Effects Against Human Cancer Cell Lines”Description:This dataset contains the raw data and original gel images underlying the findings reported in the above manuscript. Included are:S1 Raw Data: Antioxidant assay values (DPPH, ABTS, H₂O₂, NO, superoxide, total antioxidant capacity).S2 Raw Data: Cytotoxicity results from MTT and SRB assays (cell viability percentages, IC₅₀ values for HeLa, A549, and HepG2).S3 Raw Image: Uncropped DNA fragmentation gel images underlying Figure 5.S4 Raw Data: Farnesyl transferase assay kinetic readings.S5 Supporting Figures: Full spectral characterization data (IR, ¹H NMR, MS) of synthesized chalcones (CFMPY-2, 4, 15, 17, 28).All files are provided in their original format without manipulation. These data ensure transparency, reproducibility, and compliance with PLOS ONE’s data-sharing policy.Keywords: chalcones, pyrimidine derivatives, antioxidant assays, cytotoxicity, HeLa cells, A549 cells, DNA fragmentation, farnesyl transferase, apoptosis