Role of Chromogranin A on renal function in a mouse model of failing kidney

Chromogranin A (CHGA) is elevated in plasma of patients with renal failure and several CHGA polymorphisms are associated with hypertensive renal disease. Therefore, we investigated the role of CHGA in kidney disease using the partial nephrectomy model of kidney failure. Mice under surgery to remove 2/3rds of the left kidney and the entire right kidney. Control mice received the sham surgery. Wild-type and CHGA-/- mice used. To model the changes in vitro, CRL-1927 kidney cells were treated with or without CHGA in vitro. WT and CHGA-/- mice underwent sham or 5/6ths nephrectomy and were followed for 10 weeks. At the end of the study, tissue was harvested and RNA extracted for expression profiling by microarray on Nimblegen mm9 12x135K array. CRL-1927 kidney cells were treated with or without CHGA in vitro, and RNA extracted for expression profiling by microarray on Nimblegen mm9 12x135K array.