Basophils play a protective role in the recovery of skin barrier function from mechanical injury in mice

Mechanical skin injury promote an acute inflammation that impact the skin barrier function. The mechanisms to restore skin barrier function are not fully understood Overall design: After shaving the back of Balb/c mice was tape stripped 6 times with the help of a tegaderm. After 24h skin biopsy were collected and samples were stored in RNAlater. Total skin RNA was isolated with RNeasy Mini Kit (Qiagen), followed by cDNA synthesis using the SuperScript VILO cDNA Synthesis Kit (ThermoFisher Scientific). The Ion AmpliSeq Transcriptome Mouse Gene Expression Kit was used to prepare bar-coded libraries and sequenced by using an Ion S5 next-generation sequencer. The AmpliSeqRNA plug-in (ThermoFisher Scientific) was used to calculate differential gene expression analysis