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Bulk transcriptomic profiling of dermal macrophage subtypes

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NIAID Data Ecosystem2026-05-02 收录
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Viable mouse dermal macrophage (CD45+F4/80+SiglecF-) subtypes were identified by antigen expression combination of Ly6C, FRb and CD163 and sorted from dorsal skin of 7 week-old C57BL/6 female mice by FACS. Samples were paired within individual mice (5 mice each). RNA from 500 cells were isolated and profile for transcriptome by sequencing. Dermal macrophage subtypes were sorted by surface antigens by flow cytometry followed by transcriptomic profiling with bulk RNA-seq.

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2024-12-26
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