Sedimentation velocity was performed as described in Materials and Methods. Concentrations of Rep40 used were 18, 36, and 54 µM and for Rep68ΔN200: 4, 8, 16 µM.
Raw data: Sedimentation coefficient distribution of Skl WT, C31A, C36S, C125S and C36S‒C125S mutants in the absence and presence of 300 mM choline (PB, pH 6.5, 20 °C)