S100A7 expression is inhibited by curcumin in a time-dependent manner.

(A) IC50 of curcumin was detected using MTT assay for HaCaT cell survival rate after 24 h of incubation with increasing dosage of curcumin. (B) ROS analysis was measured after 16 h of incubation with increasing dosage of curcumin. (C) Western blotting using specific antibody against S100A7 revealed that the S100A7 expression in HaCaT keratinocytes was inhibited in a time-dependent manner after treatment by curcumin. Results (A, B and C) are representative of two independent experiments. All p values less than 0.05 were considered statistically significant. * p<0.05; **p<0.01; ***p<0.005.