MET dimers autophosphorylate

The activated MET receptor autophosphorylates on four tyrosine residues. Two tyrosines, Y1234 and Y1235 are located in the kinase domain of MET and their phosphorylation increases the catalytic activity of MET. Y1235 is the major phosphorylation site (Ferracini et al. 1991, Longati et al. 1994, Rodrigues and Park 1994). The other two MET tyrosines that undergo autophosphorylation are Y1349 and Y1356. These two tyrosines are at the C-terminus of MET and serve as docking sites for binding of MET effectors (Ponzetto et al. 1994, Weidner et al. 1995). It is uncertain whether tyrosine residue Y1365 is also autophosphorylated.

激活的MET受体在四个酪氨酸残基上发生自磷酸化。其中两个酪氨酸，Y1234和Y1235，位于MET的激酶结构域中，其磷酸化作用可增强MET的催化活性。Y1235是主要的磷酸化位点（Ferracini等，1991年，Longati等，1994年，Rodrigues和Park，1994年）。另外两个经历自磷酸化的MET酪氨酸为Y1349和Y1356。这两个酪氨酸位于MET的C端，并作为MET效应物结合的停靠位点（Ponzetto等，1994年，Weidner等，1995年）。至于酪氨酸残基Y1365是否也发生自磷酸化，目前尚无定论。