Pubching spatial transcriptomics of MDA-MB-231 xenografts

Gene expressional analysis with single cell scale by next generation sequencer revealed clonal dissemination in cancer metastasis. To reveal expressional heterogeneity and cell-cell interaction in the primary tumor and the metastasis, we performed transcriptome analysis of micro-tissues dissected from triple negative breast cancer (TNBC) cell line MDA-MB-231 xenograft model by our automated tissue micro-dissection punching technology. This “multiple micro-tissue transcriptome analysis” revealed that there existed three clusters in primary tumor and axillary lymph-node metastasis, two of which were cancer stem cell-like clusters (CD44/MYC-high, HMGA1-high). To reveal expressional heterogeneity and cell-cell interaction in the primary tumor and the metastasis, we performed transcriptome analysis of micro-tissues dissected from triple negative breast cancer (TNBC) cell line MDA-MB-231 xenograft model by our automated tissue micro-dissection punching technology.