Exploring the Sublethal Impacts of Cu and Zn on Daphnia magna: A Transcriptomic Perspective

Daphnia magna transcriptomic responses to IC5 levels of Cu and Zn in environmental water obtained from a pristine lake with adjusted water hardness. The study was carried out to gain insights into the Cu and Zn-regulated stress response mechanisms in Daphnia magna at the transcriptome level. RNA was extracted using Direct-zol Kit according to the manufacturer's instructions. Library preparation was carried out by purification of poly-A containing mRNA, mRNA fragmentation, random primed cDNA synthesis, adapter ligation, and adapter-specific PCR amplification by INVIEW Transcriptome Discover. Illumina paired-end read sequencing was carried out to generate 2x150 bp reads.