Proteomic analysis of CAF secretome under hypoxia vs normoxia conditions

This study investigated the differences in secreted proteins from cancer-associated fibroblasts (CAF) cultured under hypoxic and normoxic conditions. Culture supernatants from three CAF cell lines cultured under hypoxia and three CAF cell lines cultured under normoxia were analyzed by shotgun proteomics using LC-MS/MS to identify and quantify differentially expressed proteins.