lncRNA/mRNA expression profiling of miR-200c-overexpressing MDA-MB-231 cells

MiR-200c is a well-studied miRNA that is involved in stemness, the epithelial-mesenchymal transition, chemoresistance, radioresistance, and invasion/metastasis of various cancer cells. To obtain an overview of the lncRNA/mRNA regulated by miR-200c signaling in breast-cancer cell lines, we performed global lncRNA/mRNA-expression profiling on MDA-MB-231-pGIPZ and MDA-MB-231-miR-200c cells. Three independent cell-culture replicates from the total population of MDA-MB-231-pGIPZ and MDA-MB-231-miR-200c stable transfectants were used to generate total RNA. Total RNA was extracted using Trizol.