Allele and haplotype frequencies of the Czech Graves´disease patients

We
analyzed the HLA-DRB1, HLA-DQA1 and HLA-DQB1 allelic groups in 206
retrospectively observed Czech patients who had been diagnosed with Graves' disease according to
hormonal profile (low/suppressed TSH
with simultaneously elevated freeT4, freeT3, presence of thyrotropin receptor antibodies (TRAK)) as well as sonographic examination of the thyroid gland. Patients were recruited during the years 2017-2020 mainly from the
outpatients of the Institute of Endocrinology in Prague. These
patients are continuously monitored at
the Institute, which gives us detailed long-term or even lifelong retrospective
information on the status of their disease. Next generation
sequencing performed on a MiSeq (Illumina) was used for haplotyping. In the
three HLA genes (DRB1, DQA1 and DQB1), exon 2 was amplified, since this part of the HLA molecule
determines its antigenic properties.
Amplification of DNA sequences was performed using certified kits (CE 0197, CE
IVD) of allele-specific pre-coated PCR strips PROTRANS S4 HLA-DRB1 * (cat. No.
34 04), PROTRANS S3 HLA-DQB1 * (cat. No. 33 09) and PROTRANS S2 HLA-DQA1 *
(cat. No. 32 08) from the manufacturer PentaGen, Protrans, Germany.