Data underlying the manuscript: “Modular, synthetic chromosomes as new tools for large scale engineering of metabolism”

These data related to the manuscript Modular, synthetic chromosomes as new tools for large scale engineering of metabolism” included as chapter 4 of Else-Jasmijn Hassing's PhD thesis entitled “Metabolic engineering of Saccharomyces cerevisiae for the production of aromatic compounds”.<br><br>The construction of powerful cell factories requires intensive genetic engineering for the addition of new functionalities and the remodeling of native pathways and processes. The present study, using <i>Saccharomyces cerevisiae</i>, explores the potential of modular, specialized <i>de novo</i>-assembled neochromosomes (named NeoChrs) to facilitate extensive genome reprogramming. Linear and circular NeoChrs carrying 20 native and 21 heterologous genes were designed and constructed by <i>in vivo</i> assembly in yeast in two transformation steps, leading to <i>de novo</i> production of anthocyanins, native to plants. Turned into exclusive expression platforms for heterologous and essential metabolic routes, the NeoChrs behaved like native chromosomes regarding mitotic and genetic stability, copy number, harmlessness for the host and editability by CRISPR-Cas. This study paves the way for future microbial cell factories with modular genomes in which core metabolic networks, localized on satellite, specialized NeoChrs can be swapped for alternative configurations and serve as landing pads for addition of functionalities.

本数据集关联的是收录于Else-Jasmijn Hassing题为《用于芳香化合物生产的酿酒酵母（Saccharomyces cerevisiae）代谢工程》的博士论文第4章的手稿《模块化合成染色体作为代谢大规模工程化的新型工具》的相关数据。

构建高性能细胞工厂需通过高强度遗传工程操作，以引入全新功能并重塑天然代谢途径与生理过程。本研究以酿酒酵母（Saccharomyces cerevisiae）为实验材料，探究了模块化定制化从头组装（de novo）新染色体（NeoChrs）在实现大规模基因组重编程方面的应用潜力。研究中通过两步转化步骤在酵母体内（in vivo）完成组装，设计并构建了分别携带20个内源基因与21个外源基因的线性及环状新染色体（NeoChrs），实现了植物源花青素（anthocyanins）的从头合成。将新染色体（NeoChrs）改造为外源功能与核心代谢途径的专属表达平台后，其在有丝分裂与遗传稳定性、拷贝数、对宿主的无害性以及CRISPR-Cas编辑可行性等方面均表现出与内源染色体一致的特性。本研究为未来构建模块化基因组的微生物细胞工厂开辟了道路：这类基因组中，定位于卫星型定制新染色体（NeoChrs）的核心代谢网络可被替换为其他构型，并可作为功能引入的着陆平台。