Data From: Matrix tropism influences endometriotic cell attachment patterns

Due to the extended period for clinical diagnosis, the etiology of endometriotic lesion initiation is not well understood or characterized. Endometriotic lesions are most often found on pelvic tissues and organs, especially the ovaries. To investigate the role of tissue tropism on ovarian endometrioma initiation, we adapted a well-characterized polyacrylamide microarray system to investigate the role of tissue-specific extracellular matrix and adhesion motifs on endometriotic cell attachment, morphology, and size. We report the influence of cell origin (endometriotic vs. non-endometriotic), substrate stiffness mimicking aging and fibrosis, and the role of multicellular (epithelial-stromal) cohorts on cell attachment patterns. We identify multiple ovarian-specific attachment motifs that significantly increase endometriotic (vs. non-endometriotic) cell cohort attachment that could be implicated in early disease etiology. , (As per described methods in the manuscript)  Details on cell culture, Polyacrylamide (PA) microarray fabrication, and PA microarray experimentation were provided in the methods section of the associated paper. Slides were immunostained for actin and cell nuclei, cells prior to seeding were treated with Cell Tracker. Cellular microarrays were imaged at 10x and 20x magnification on the Zeiss AxioScan.Z1 Slide Scanner. Images of entire arrays were converted to 8-bit TIFF files per output channel using Fiji (ImageJ) and cropped into single island images utilizing dextran-rhodamine marker islands to identify the location of arrayed conditions within each image. CellProfiler (version 4.2.6) was used to identify and measure nuclei and fluorescent regions using the IdentifyPrimaryObjects, IdentifySecondaryObjects, and MeasureObjectSizeAndShape modules. Single-cell fluorescent intensity was quantified using the MeasureObjectIntensity module. The output from CellProfiler was loaded into RSt..., , # Data From: Matrix tropism influences endometriotic cell attachment patterns

[https://doi.org/10.5061/dryad.w6m905r0b](https://doi.org/10.5061/dryad.w6m905r0b)

## Description of the data and file structure

(As per described methods in the manuscript)   

Endometriotic or non-endometriotic cells were cultured as described.  PA Microarrays were fabricated as previously described.  

### Files and variables

For Cell Profiler Output files, there are many columns included in the CSV, this is the raw output from our analysis pipeline in Cell Profiler analyzing the cropped images of the slides included in this data. 

A brief explanation of these files are included below:

* ImageNumber & ObjectNumber - Metadata associating measurements with specific image and object 
* Columns Starting with \"Metadata_\" - Metadata for future processing including Cell Type, Experiment, Scene, Row and Column.
* Columns Starting with \"AreaShape_\" - These are measurements from the MeasureObjectSizeShape modul..., The cell lines used in this work were originally obtained from human subjects by other researchers and were de-identified at that time. There are no human subjects in this work as we used previously de-identified cell lines.