Analysis of mRNA expression in CHAPIR overexpressed heart

To elucidate the molecular mechanism by which cardiac-hypertrophy-associated piRNA (CHAPIR) regulates gene expression, RNA-seq was performed on control or CHAPIR-overexpressing mice heart. Based on RNA-seq data, 720 differentially expressed genes were shown in CHAPIR-overexpressing heart relative to NC control, including 519 upregulated and 201 downregulated genes. Overall design: Total RNAs were extracted from CHAPIR mimic or mimic-negative control (NC) overexpressed heart. Then, high throughput RNA sequencing was performed to identify differences in the gene expression profile.