File S1 - Kinetic Titration Series with Biolayer Interferometry

Supporting files. Figure S1, Repetitions of the kinetic titration series. A–F) Measurements are indicated in black and the corresponding fit by a red line. Below each sensorgram is a plot of the respective fit residuals. Figure S2, Repetition of the parallel sensor kinetics. The sensorgrams are indicated by blue lines in different darkness and the corresponding fits by red lines. Below are the plots of the respective fit residuals given in the same blue as above. Figure S3, Comparison of the fitting models without use of the RI term. A) Fit of parallel sensor kinetics without RI and residual plots below. B) Fit of kinetic titration series without RI and the respective residual plot below. Method S1, Preparation of protein G B1. Table S1, Comprehensive table of all evaluated fits. F1A/B: Fitting results for the measurements illustrated in Fig. 1. S1A–S1F/S2/S3: fitting results for the measurements illustrated in Fig. S1, Fig. S2 and Fig. S3. ka: on-rate constant, kd: off-rate constant, KD: dissociation constant (kd/ka), RI1–RI5: baseline drift in nm, Χ2: chi2 in nm2. Script S1, Example script (Python) for combining BLI raw data. This example script illustrates how to combine the raw data (after export) from the ForteBio software to a unified single cycle kinetic for import by third party software. Script S2, Residual calculation of kinetic titration series. Script to calculate a residual table from the exported fits based on the measurements after data export in a straight forward way. (DOCX)