Bacteroides phages recovered from human and animal faecal matrices in rural Kenya

This data describes the recovering and isolation processes of Bacteroides spp. strains from human and cattle faecal sources from rural areas in Siaya County (Kenya), and occurred between 7th and 28th of June 2018. The data also includes the detection of bacteriophages (infecting these Bacteroides spp. host strains) in conjunction with traditional faecal indicator organisms in water sources from Kisumu and Siaya County (Kenya) occurring between June 18th 2018 and June 13th 2019. Exact location (coordinates) of the sample points are also described in the data set. A microbiological technique using Bile Esculin Bacteroides (BBE) agar was used for the recovering and isolation processes of Bacteroides spp. strains. Standard ISO (7899-2, 9308-1, 10705-2 and 10705-4) techniques, such as membrane filtration and the double-agar-layer methods, were used for the detection of bacteriophages and traditional faecal indicator organisms. The purpose of data collection was to develop new markers that could identify cattle and/or human sources of faecal contamination, which could be used as part of a Microbial Source Tracking (MST) tool box. Technicians and researchers from the University of Brighton (UK), University of Southampton (UK), from the Victoria Institute for Research on Environment and Development (VIRED) (KE) and from the Kenya Medical Research Institute (KEMRI) (KE) were responsible for the collection and interpretation of data.

本数据集详细记录了从肯尼亚西雅县（Siaya County）农村地区人类及牛粪源中分离出的拟杆菌属（Bacteroides spp.）菌株的恢复和分离过程，该过程发生于2018年6月7日至28日。数据还包括了在肯尼亚基苏木（Kisumu）和西雅县（Siaya County）水源中检测到的噬菌体（感染这些拟杆菌属宿主菌株的病毒）以及传统粪便指示微生物，检测时间跨度为2018年6月18日至2019年6月13日。数据集中还描述了采样点的精确位置（坐标）。在恢复和分离拟杆菌属菌株的过程中，采用了胆汁麦芽糖拟杆菌（Bile Esculin Bacteroides，简称BBE）琼脂的微生物学技术。为了检测噬菌体和传统的粪便指示微生物，使用了符合ISO标准（7899-2、9308-1、10705-2和10705-4）的技术，如膜过滤和双层琼脂层法。数据收集的目的是开发新的标记物，以识别粪便污染的牛或人类来源，这些标记物可作为微生物源追踪（Microbial Source Tracking，简称MST）工具箱的一部分。数据收集和解读由布莱顿大学（英国）、南安普顿大学（英国）、维多利亚环境与发展研究所（Victoria Institute for Research on Environment and Development，简称VIRED）（肯尼亚）和肯尼亚医学研究研究所（Kenya Medical Research Institute，简称KEMRI）（肯尼亚）的技术人员和研究人员负责。