List of primers selected for the real-time PCR array based on their specificity and sensitivity with in-house analytical panels.

a- to simplify the process of evaluation we used our primer names with sequential numbers, however some of the primers have been designed previously with their original names in the articles listed in the last column of this table;b- variants’ coverage is based on multiple nucleotide sequence alignments performed “in house”;c- analytical sensitivity here is based on testing with in-house DNA standards;d– primers were selected from sited studies or designed “in-house” using DNA/RNA signatures generator “Insignia” (Ins.) or comparative genome analysis (CGA); -m – modified; Sub. – subtypes; Gen. – genotypes; Excl. – excluding; M (A or C), R (A or G), K (G or T), Y (C or T), N (A or G or T or C), D (A or G or T); HA – Haemagglutinin, *VACV genome annotation is according to reference [33].