A unique population of regulatory T cells in heart potentiates cardiac protection from myocardial ischemia/reperfusion injury

Purpose: The aims of the study were to identify that transctiptome of heart Tregs was different from lymphoid organ Tregs. Methods: Tregs from injured hearts and paired lymphoid organs of the Foxp3-GFP transgenic mice were double-sorted by magnetic-activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) to generate RNA-sequence 3 days after myocardial ischemia/reperfusion injury (I/R injury). Results: Transcriptional profiling revealed that the transcriptome of heart Tregs has unique characteristics when compared to lymphoid Tregs.